Aioi A, Watanabe R
SPTM R&D Australia、Eskitis Institute Griffith University
Pham N, Quinn R
Eskitis Institute Griffith University

[Abstract]
Dodonaea, a genus of 70 species in Sapindaceae, has a cosmopolitan distribution in tropical and subtropical regions, especially in Australia. Here, we examined the anti-oxidant effect of the extract of Dodonaea Sapindaceae (DS). In the assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH, a stable free-radical molecule), 200µge (microgram equivalent)/ml of DS scavenged radicals to the same extent as 50µg/ml ascorbic acid. Further cell-free assay systems showed that DS had superoxide dismutase (SOD)-like activity, but not catalase-like activity. Hydrogen peroxide (H2O2)-induced intracellular reactive oxygen species (ROS) in THP-1, a human acute monocytic leukemia cell line, was suppressed by pre-treatment with 200µge/ml of DS, while there was no significant cytotoxicity. Nitric oxide (NO) production from RAW264.7, a murine macrophage-like cell line that is stimulated by lipopolysaccharide (LPS), was also suppressed by addition of 200µge/ml DS. These results suggest that DS can be an anti-oxidant active ingredient for cosmetic applications